TY  - JOUR
AU  - Jiayi Yang
AU  - Pei Qun
AU  - Lida Zhang
AU  - Juan Lin
AU  - Xuanwei Zhou
PY  - 2025
DA  - 2025/04/28
TI  - Response Surface Methodological Approach for Optimizing the Expression of Recombinant Ganoderma leucocontextum Immunomodulatory Protein in Pichia pastoris
JO  - Japan Journal of Research
VL  - 6
IS  - 6
AB  - Ganoderma leucocontextum immunomodulatory protein 2 (FIP-gle2) is a recently identified small molecule
protein. Based on the successful expression of FIP-gle2 in Pichia pastoris GS115, this study optimized the
expression conditions of the engineered P. pastoris using response surface methodology (RSM). Initially,
single-factor experiments determined that the optimal parameters for subsequent optimization were a
cultivation period of 4 days, a temperature range of 26 to 30 °C, an initial pH of 6.0 to 7.0, and a methanol
(MeOH) concentration of 0.75% to 1.25%. Subsequently, a box-behnken design (BBD)-based RSM was
employed, with the total protein concentration in the fermentation broth serving as the detection indicator.
The optimal fermentation conditions were determined to be a cultivation temperature of 27 °C, an initial
pH of 6.7, and a MeOH concentration of 1.11%. Under these conditions, the actual operational parameters
were set at a fermentation temperature of 27 °C, an initial pH of 6.5, and a MeOH concentration of
1%, resulting in an average total protein concentration of 225.12 mg·L-1. This study provides preliminary
optimization of the cultivation conditions for P. pastoris, offering valuable data support for the industrial
production of this protein.
SN  - 2690-8077
UR  - https://dx.doi.org/10.33425/2690-8077.1184
DO  - 10.33425/2690-8077.1184