TY - JOUR AU - Verónica D Hurtado-Narváez AU - Nidia E de León-Frías AU - Catalina Méndez-De la Espriella AU - Adriana Rodríguez-Ciodaro AU - Oscar E Gomez-Cárdenas AU - Hugo Díez-Ortega PY - 2025 DA - 2025/07/04 TI - Exploring Genetic Diversity in Enterococcus Faecalis Strains of Endodontic Origin: Pilot Study JO - Japan Journal of Research VL - 6 IS - 7 AB - Objective: The variability in reports associating Enterococcus faecalis with endodontic treatment failure is due to its remarkable genetic plasticity and clonal diversity facilitating the adaptation to adverse microenvironments. Materials and Methods: Present study approximated the genetic diversity of Enterococcus faecalis isolates from oral cavity samples of endodontic origin; the detection involves genes Asa, Esp, EfaA, Ace, Asa373, GelE, and CylA, and mature biofilm formation. Results: On the other side, the genetic clusters were analysed based on the sequence alignment of the amplified product of a 16S rDNA PCR. Results showed that the genetic clustering of Enterococcus faecalis isolates was distinct between patients but with a high degree of similarity between samples from the same individual. Conclusion: The virulence genes presence and biofilm formation resulted independent of genetic clustering but related to sample origin. SN - 2690-8077 UR - https://dx.doi.org/10.33425/2690-8077.1193 DO - 10.33425/2690-8077.1193