Objective: The variability in reports associating Enterococcus faecalis with endodontic treatment
failure is due to its remarkable genetic plasticity and clonal diversity facilitating the adaptation to
adverse microenvironments.
Materials and Methods: Present study approximated the genetic diversity of Enterococcus faecalis
isolates from oral cavity samples of endodontic origin; the detection involves genes Asa, Esp, EfaA, Ace,
Asa373, GelE, and CylA, and mature biofilm formation.
Results: On the other side, the genetic clusters were analysed based on the sequence alignment of the
amplified product of a 16S rDNA PCR. Results showed that the genetic clustering of Enterococcus
faecalis isolates was distinct between patients but with a high degree of similarity between samples from
the same individual.
Conclusion: The virulence genes presence and biofilm formation resulted independent of genetic
clustering but related to sample origin.
